The Threat of Carbapenem-Resistant Non-Fermenting Gram Negative Bacteria (NFGNB)

Aim: To Isolate Non fermenting Gram negative bacteria (NFGNB) from clinical specimens of infected patients in a tertiary care hospital and then determine the antimicrobial susceptibility these isolates to Carbapenems Methodology: It was Retrospective Cross-Sectional study conducted department Pathology, hospital, Lahore December 2020-November 2021. The included 4320 received Microbiology lab, for culture sensitivity consisted urine, sputum, bronchial washings, pus, wound swabs, ETT, Folly’s catheter tip, HVS, body fluids, blood etc. All other were inoculated on Blood agar (Oxoid UK), Mac Conkey urine CLED UK). plates incubated overnight at 37°C. Bacterial identification achieved by gram staining, biochemical profile. Analytical profile index API-20NE (Biomerieux, France) used non (NFGNB). Results: Four thousand three hundred twenty processed isolated 651 cases constituting 15.1%. Out GNR, 130(19.97%) NFGNB isolated. Maximum number obtained admitted Surgery ward 46 followed Medical consisting 38 cases, 15 each ICU OPD. Pus/wound swabs yielded majority 58(44.6%), 33(25.3%), sputum 15(11.5%), HVS 14(10.7%). Pseudomonas aeruginosa exhibited 35.29% 32.35% resistance meropenem (MEM) imepenem (IPM) respectively. Conclusion: are emerging pathogens responsible variety infections. These resilient have developed alarming against previously considered last therapeutic resort, carbapenems. Keywords: NFGNB, Carbapenems, Kirby Bauer disc diffusion method, CLSI, meropenem, imepenem,